In this paper a new test method for determining transmissibility of an aquifer in well is described. Using a test of step change of air pressure at well head, the transmissibility of an aquifer in Liaocheng city is determined to be 14.4m2/d. But it was determined by pumping test to be 43.6m2/d. Both the data have the same order of magnitude. This test is more simple and convenient, more economical and faster than the pumping test. It is also more reasonable to explain the micro-regime of groundwater and easier to be used in practice.